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In summary, to enable further in silico analysis, the semaglutide API peptide sequence is modified to H A EGTFTSDVSSYLEGQAA Z EFIAWLVRGRG

The potential risks associated with GLP-1 injections include gastrointestinal side effects like nausea, vomiting, diarrhea, and constipation

Experimental studies suggest it may also have mild anticoagulant effects by inhibiting clotting factors

This may result in a false negative result as the media containing the LDH was replaced with fresh serum-free media and incubated for another 24 h and most of the LDH released into the media would have been removed in the process

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