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Mechanistic pathways associated with acetaminophen-induced hepatotoxicity APAP is rapidly absorbed and extensively metabolized upon entering the liver, with 8590% conjugated with glucuronic acid or sulfate by the enzymes uridine diphosphate-glucuronosyltransferase and sulfotransferase and subsequently eliminated

Gur et al

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To measure the reverse reaction rates, we mixed a pre-equilibrated mixture of RT (20 M) and GSH (10 or 5 mM) with an equal volume of PBS and followed the disappearance of RT-GSH fluorescence (Fig