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Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer

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A broad spectrum of antidepressant drugs, such as selective serotonin reuptake inhibitors (SSRI), tricyclic antidepressants, selective dopamine reuptake inhibitors, and selective norepinephrine reuptake inhibitors (SNRI), is introduced to change serotonergic, dopaminergic, glutamatergic, or noradrenergic activity levels in the central nervous system (CNS) (Harmer, Duman, and Cowen, 2017)

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