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The dissected gonads were washed three times for at least 30 min (10-min interval) with 1x PTW/0.5%BSA solution and incubated the dissected gonads in the 1x PTW/0.5% BSA solution containing the fluorescence-conjugated secondary antibodies for 1-2 hours at room temperature

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Dilution itself doesn't degrade the peptide

doi: 10.1007/s12011-012-9320-1

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