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The reaction mixture was carried out in a total volume of 20 L consisting of total RNA up to 1 g, 4 L 5x Trans Amp buffer, 1 L reverse transcriptase, and DNAase-free water up to 20 L

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9711, otherwise known as the Food and Drug Administration Act of 2009, the manufacture, importation, exportation, sale, offering for sale, distribution, transfer, non-consumer use, promotion, advertising or sponsorship of health products without the proper authorization from FDA is prohibited

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