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Postgrad Med J 1992

TNF Inhibition for Immune Checkpoint Inhibitor-Induced irAEs: The Jury is Still Out

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Plateable PHHs Hu8132 and Hu8163 (Thermo Fisher Scientific, catalog: HMCPMS) were thawed in cryopreserved hepatocyte recovery medium (Thermo Fisher Scientific, catalog: CM7000) and plated with Williams E Medium supplemented with primary hepatocyte thawing and plating supplements (Thermo Fisher Scientific, catalog: CM3000)

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Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)