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Response: As readers are aware, we have been contemplating potential clinical APC assignments for the past number of rulemaking cycles but are not convinced as to what clinical APC would be appropriate in terms of clinical and resource homogeneity

Mistake 5: Poor sterile technique Everything else can be perfect but if you skip the sterilization steps, you risk contaminating the solution with bacteria

doi:10.1128/aem.03417-12

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Some researchers might push this to 6 weeks, but we recommend exercising extreme caution beyond the 4-week mark to ensure optimal efficacy