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Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours

Patients should be prescribed these medications according to their licensed indications and dosing regimens

For localised pain, topical NSAIDs are a useful alternative that avoids most of the stomach and kidney risks

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Basal insulin therapy should be considered when introducing dual or triple therapy and should be intensified adding a mealtime insulin or a GLP-1 RA when glycemic control needs to be optimized [1]