The forward and reverse oligonucleotides of this fragment were, respectively, 5- GATCT ATGCTGTTTAATCTGAGGATCCTGTTAAACAATGCAGCTTTTAGAAATGGTCACA ACTTCATGGTTCGAAATTTTCGGTGTGGACAACCACTACAAG-3 and 5- AATTC TTGTAGTGGTTGTCCACACCGAAAATTTCGAACCATGAAGTTGTGACCATTTCTA AAAGCTGCATTGTTTAACAGGATCCTCAGATTAAACAGCATA-3 ( Bgl II and Eco RI sites underlined
After three washes with TBST, membranes were incubated with HRP-conjugated secondary antibodiesGoat anti-Rabbit IgG H&L (HRP, Cat
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