These treatment groups included a GSH group (0.1 g/kg), a GO@GSH group (equivalent to 0.1 g/kg GSH), and a GO@GSH-FA group (equivalent to 0.1 g/kg GSH)
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There are at least five different mechanisms by which vitamin E exerts its antioxidant functions
LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
This leads carbon 3 to form a double bond with oxygen and to lose a hydrogen
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