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Declaration of Competing Interest The author declares that he has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper

Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

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After pregnancy confirmation, liraglutide was discontinued and insulin therapy was started