The deviation from linearity is probably due to geometric constraints in the roGFP barrel similar to those described for roGFP1-iR which had been expected to be less reducing than roGFP1-iL due to stabilization of the thiolate anion by the introduced adjacent basic arginine residue (Lohman and Remington, For live cell measurements biosensors should ideally exhibit a strong preference for one specific analyte
Group IV (Control-combination) was administered PBS for 3 days, then orally given a combination therapy of GABA (200 mg/kg/day for 21 days), probiotics (Fiber probiotic, Proflora, 0.2 g/kg/day for 21 days), vitamin D3 (SUN-D 1000, California Greens, 1,000 IU/kg/day for 14 days) and -lactam (Amoxicillin, HYMOX, 200 mg/kg/day for 7 days) as described in previous studies (Bin-Khattaf et al., 2022
doi: 10.1007/bf02360196 7 AhmadRHaldarC
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