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The equilibrium dissociation constant ( K D ) was derived via the following formula: K D = k off (dissociation rate, s 1 )/ k on (association rate, M 1 s 1 )

B 197 , 111534 (2019)

Piperidine, diisopropylcarbodiimide (DIC), N -hydroxybenzotriazole (HOBt), MPAA (97%, MPAA), 3-mercaptopropionic acid (MPA), tris(2-carboxyethyl)phosphine hydrochloride (98%, TCEPHCl), thiophenol, triisopropylsilane (TIS), guanidine hydrochloride (99%, GnHCl), hydrochloric acid (reagent grade, 37%), and sodium hydroxide (pellets, 97%) as well as other reagents were purchased from Sigma Aldrich, Acros Organics or Merck and were of the purest grade available

Irg1 is a mitochondrial enzyme that produces itaconates

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Li C, Yuan J, Zhu YF, Yang XJ, Wang Q, Xu J, He ST, Zhang JA