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Briefly, cells were fixed, processed, mounted on Epon stubs, polymerized at 60 C, and 70 nm sections cut en face with a diamond knife (DiATOME) in a Leica Ultracut UCT ultramicrotome before examination on an FEI Tecnai G2-Spirit TEM

Samples were delivered on dry ice for analysis

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HIF-1 downregulation increases ACSL4 to promote ferroptosis, and Nrf2 mediates HIF-1 activation to alleviate ischemic AKI (102)

Most ZMA supplements are dosed similarly to the amounts listed above

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