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The following validated biomarkers offer clear insight into these peptide-linked responses: Collagen type I and III levels: These markers are measured through fluorescence or staining methods and indicate structural changes in the extracellular matrix, offering a reliable way to track collagen deposition and remodelling throughout controlled laboratory experiments

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Table 2 summarizes GHKs effects on genes relevant to cell cycle regulation

Junction PCR confirmed the knock-in event in the liver, and digital PCR indicated an efficiency of approximately 1% for a single administration of Donor+Cas9, consistent with previously reported in vivo knock-in efficiencies (Fig

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