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This notable behavioral enhancement was supported by NACs role in restoring essential proteins for glutamate homeostasis in the NAc after a week of treatment, and significantly, by elevating the expression of type II metabotropic glutamate receptors (mGluR2/3) after 50 days

They were then spun down, removed of their supernatant, and resuspended and washed with several changes of PBS, then washed over night in 5 mL PBS

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