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To decipher how DDX3X might promote the fusion of perinuclear virions with the outer nuclear membrane, we considered probing the tegument content of perinuclear virions, but this would require their purification, a difficult task only previously achieved by one lab 87

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Cells were finally washed three times using 1X PBS and were mounted on glass slides in Dako containing 0.1 g/mL Hoechst 33342 (Sigma-Aldrich)

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PubMed Central, doi:10.1371/journal.pone.0062442.Beuten, Joke, et al