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For cystine starvation, DMEM lacking glutamine/methionine/cystine (Thermo Fisher Scientific) with glutamine (Thermo Fisher Scientific) and methionine (Sigma Aldrich) supplement was used as a base cystine-deficient culture medium

The result that Pdcd4 knockdown increased the distribution of p70s6k1 mRNA but not p70S6K2 mRNA in the polysomal fractions indicates Pdcd4 inhibiting p70S6K1 translation (Fig

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J Pediatr 102 : 931935

B12 primarily supports nerve health, brain function, and energy production

the images were acquired again after 48 h of incubation at 37 C