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The protein samples (12 L) and Spectra Multicolor Broad Range Protein Ladder (7.5 L) with 10260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1 Bolt MES SDS Running Buffer (Invitrogen)

However, formation of glial scar via the accumulation of reactive astrocytes is a major obstacle to axonal regeneration by neurons after injuries (Zhang and De Koninck, 2006

You will see it stated confidently in a lot of places that the body adapts after 4 to 6 weeks, tolerance builds, and the effect fades, so you have to cycle. It sounds authoritative

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