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Centrifuge anticoagulant-treated blood at 1,000 g for 10 min at 4C

Homocysteine can be converted back to methionine in a reaction catalyzed by vitamin B 12 -dependent methionine synthase, which requires 5-methyltetrahydrofolate (5-meTHF) as a methyl donor

and 424.205(f)(5) to update requirements for achieving 5 and 9 percent weight loss measured in accordance with 410.79(c)(ii)

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The dorsal putamen was selected as the center of the volume of interest due to its relatively homogenous mix of neurons and astrocytes, and suitable distance from bone and other regions that could compromise signal quality

On days 1, 3 and 5 of the study, animals were water deprived overnight before presentation of two bottles containing the same novel flavoured solution (either cherry or grape, plus sucralose)