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Freeze-thaw cycling is especially destructive to peptides each cycle forms ice crystals that physically shear the molecule, so even one accidental freeze can tank a vial

Weve looked at how different health issues and how well your body absorbs B12 affect how often you should get shots

Chen, D.Y

Science 300(5619):644647

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(86) showed that castration of male rats lowered the levels of CAT, SOD, GPx, and GR in the left ventricle of the heart, as well as GSH and protein-thiol groups, and increased lipid peroxidation and nitrotyrosine concentrations