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Flow cytometry Flow cytometry (CytoFLEX, Beckman Coulter, Inc., Miami, FL, USA) was used to measure apoptosis and ROS levels in testicular cells following a previously described method [54]

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Quantitative Real-Time PCR (qRT-PCR) Total RNA was extracted from homogenized liver tissue using the TRI reagent and Direct-zol RNA Miniprep Kit (ZYMO Research, USA)

Dub M, Tastet O, Dufour C, Sannier G, Brassard N, Delgado GG, et al