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Assessing the quality of reports of randomized clinical trials: is blinding necessary

10.1136/rmdopen-2022-002600 9

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the antibody (R1401-12) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system

The effects typically last about one week, depending on your bodys needs and overall health

The pace of weight loss typically plateaus around months 46, then continues at a slower but steady rate through months 612