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In addition, the increase in cellular levels of TNF- and IL-1 has been related to the decrease in occludin expression and ZO-1 and 2 in the hypoxic brain (Rochfort and Cummins, 2015

Half an hour before the end of the incubation with the indicated stimulus, Hoechst was added to each well of 24-well plates at a final concentration of 1 M

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