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Notably, recent work from our own laboratory has demonstrated that alongside proteins that are either gained and/or modified during post-testicular sperm maturation, the shedding of proteins also constitutes a major part of the proteomic changes that contribute to producing a fertilization competent sperm cell (57, 109)

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The loss of enterohepatic recirculation would definitely cause an additional loss of 5mcg of B12 that is not recovered from bile