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Mice were euthanized using gradual-fill CO inhalation in accordance with the AVMA Guidelines for the Euthanasia of Animals (2020), followed by cervical dislocation to ensure complete euthanasia before tissue collection

Nonetheless, there is a scarcity of clear data regarding the impact of vitamin D levels on thyroid function in healthy individuals (10)

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Additionally the study found that during the process of cell division there were fewer spindle defects and decreased abnormalities of the cortical granules when evaluated at 12 and 24 hours

At various time intervals, the GSH was measured by reaction with an excess of 5-5'-dithiobis-2-nitrobenzoic acid (DTNB)