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The thoracic aortas were surgically excised and carefully cleared of surrounding tissues

This subtracted library was added to a monolayer of Ishikawa cells grown in a 3.5-cm tissue culture plate and incubated at 37 C for 30 min, allowing bound phage to be internalized by endocytosis

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Lipotoxicity, nutrient-sensing signals, and autophagy in diabetic nephropathy