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MOTS-c Before You Start: The Critical Protocol Facts MOTS-c is a mitochondria-derived peptide that regulates cellular energy metabolism, making it mechanistically distinct from every other peptide in clinical use today

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This finding aligns with prior research showing that fasting interventions can upregulate antioxidant enzymes and reduce markers of oxidative stress 21,36

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Cell culture analyses The human colon cancer cell line HCT116 and human hepatoma cell line HuH7 were maintained in DMEM medium containing 10% FCS