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A Gene Ontology (GO) functional enrichment analysis of differentially expressed proteins (DEPs) was also carried out, as depicted in Fig

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All g-Blocks were assembled into individual DVK-AF destination plasmids using the MoClo procedure

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For instance, supplementation of immature mouse testicular cultures with 5% PRGF was associated with enhanced production of flagellated sperm and increased markers of germ cell proliferation and differentiation, such as PLZF, SYCP3, ACRBP, and Ki67, along with reduced Bax expression (34)

Each lot is tested by HPLC for purity, LC-MS for identity confirmation, and endotoxin assay for sterility readiness