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RNA interference To silence GLP-1R and PKA gene expression, cells were transfected with siRNAs using lipofectamine RNAi MAX reagent (Invitrogen)

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pylori colonization also negatively affects the expression of antioxidant proteins, along with epigenetic modifications and DNA methylation, such as GATA-4, GATA-5 and TWIST-1 (88), as well as miRNAs dysregulation, such as mir-21, mir-92a, mir-27a, mir-146a, mir-326, mir-155 and mir-663 (89)

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It is owing to the growing cosmetic industry in the Asia Pacific region, pertaining to its rising urban population and beauty trend from its neighboring regions