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Plasmid pBV220 (an ampicillin-resistant and inducible expression vector, carrying the PRPL promoter) gshI gene: Forward primer : 5-ATTTACCGcaattg ATGATCCCGGACGTATCAC-3 Reverse primer : 5 -AATTACCGcaattgTCAGGCGTGTTTTTCCAG-3 caattg is Mun site gshII gene: Forward primer : 5 -TTAACCGgtcgacAATTGCCATGACTGCCC-3 Reverse primer : 5 -TTAACCGgtcgacTTACTGCTGCTGTAAACG-3 gtcgac is SalI site gshI b gene : Forward primer : 5 -ACCGAATTCctgcagGCATTCAAAGCAGAAGGC-3 Reverse primer : 5 - ACCGAATTCctgcagTCAGGCGTGTTTTTCCAG-3 ctgcag is PstI site Plasmid PBV01: gshI gene was inserted into pBV220 plasmid treated with EcoRI to generate plasmid pBV01