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Add 2.0 mL bacteriostatic water to one 5000 IU vial 2,500 IU/mL

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For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

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