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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing
Short-Chain Acylcarnitines (C0-C5) Carnitine, Acetyl, Propionyl, Butyryl & Isovaleryl Medium-Chain Acylcarnitines (C6-C12) Long-Chain & Unsaturated Acylcarnitines (C14-C18+) LC-MS/MS Platoorm & Method oor Acylcarnitine Quantioication LC-MS/MS Platoorm SCIEX QTRAP 6500+ with scheduled MRM acquisition
They are taking therapeutic drugs to ensure their bodies dont reject their new organs, and therapeutic drug monitoring (TDM) is necessary to guard against toxicity
10.1001/jama.2023.28239 JAMA
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