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Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol

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Inhibition of kynurenine 3-monooxygenase (KMO) significantly suppressed XA elevation and attenuated both oxygen-glucose deprivation (OGD)-induced cardiomyocyte injury and ligation-induced myocardial ischemia injury, suggesting XA's potential utility as a ferroptosis marker in CAD