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As most of the available evidence stems from in vitro and preclinical studies, there remains a need for long-term and in vivo research to substantiate these findings and assess their translational relevance

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Although there is little evidence that low doses of hydrochlorothiazide (12.525 mg daily) reduce the risk of heart attack, stroke or death (Messerli et al., 2011), higher doses have been proven effective in lowering blood pressure and improving cardiovascular outcomes in patients with hypertension