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Methods: Ten-week-old Mdr2 -/- mice with congenital PSC-like progressive biliary disease, and healthy wild-type littermates (WT) mice enrolled as control fed with chow diet or DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine) were subjected to either modified retrograde biliary instillation or hydrodynamic tail vein injection of sleeping beauty transposon-transposase plasmid system with activated forms of AKT (myr-AKT) and Yap (YapS127A) protooncogenes (SB AKT/YAP1)

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