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The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 M FOXO4-DRI was added in the medium immediately

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Protocol 2: neuroprotection and recovery support Goal: Support neurological recovery after injury or provide intensive neuroprotective intervention