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Following 24 hours of treatment, cells were washed with phosphate buffered saline (PBS 1X) and stored at 80C

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Physiol Plant 107:294302 Kocsy G, von Ballmoos P, Regsegger A, Szalai G, Galiba G, Brunold C (2001) Increasing the glutathione content in a chilling-sensitive maize genotype using safeners increased protection against chilling-induced injury