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Concentrations of 10 M, 15 M and 20 M were selected for clomipramine, fluoxetine, nortriptyline, and paroxetine
Soares, 2014)
Materials and Methods Cell Culture and Drug Treatments HCM, C2C12 and HUVEC were cultured in high-glucose DMEM (Gibco BRL, USA) supplemented with 5% fetal bovine serum (Gibco BRL, USA) without antibiotics in acceptable conditions (5% CO 2 , 37C)