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Then, the RAW264.7 cells were lysed using RIPA lysis buffer supplemented with 1 mM phenylmethanesulfonyl fluoride (PMSF)
Additional critical pharmacological factors that dictate the bioavailability and efficacy of intranasal compounds include drug metabolism in the nasal cavity, degree of dissociation (pKa), chemical structure, drug half-life (t ), osmolarity and pH
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(5) IIRI + MET + Abx group: administered oral antibiotics (ampicillin 1 g/L, neomycin 1 g/L, metronidazole 1 g/L, and gentamicin 160 mg/L) combined with metformin in drinking water for one week prior to IIRI
doi: 10.1016/j.ejim.2017.06.011
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