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The oxidative arm is an irreversible mechanism associated with the production of NADPH

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A2S) and washed two times with GST-A buffer

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Equal amounts of protein (40 g) were separated by 7.5 12% SDS-PAGE and then transferred electrophoretically onto polyvinylidene difluoride (PVDF) membranes (Millipore, Bedford, MA, United States)