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Research conducted in vivo and in vitro has shown that acrolein causes oxidative stress, resulting in the rupture of cell membranes, DNA and mitochondria damage, endoplasmic reticulum (ER) stress, and the potential to initiate apoptosis (112, 113)

However, the chemical falls under the category of non-approved substances and is strictly prohibited at all times under the UFC's anti-doping policy

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Angle the needle and slowly allow water to slide down and gently contact the lyophilized material

The exosomes obtained were resuspended in PBS for subsequent experiments

There was no significant difference among the three groups in the vascular permeabilities on the non-ligature (contralateral) sides