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Introducing EBNA1-targeting gRNAs into EBV-positive CNE2 cells resulted in substantially reduced GFP fluorescent signals (Fig

After washing steps, DNA was eluted twice with elution buffer (25 mM TrisHCl pH 7.5, 5 mM EDTA pH 8.0, 0.5% SDS, 65 C, 20 min), treated with 10 mg/mL RNase-A (65 C, 1 h) and with 20 mg/mL proteinase K (65 C, 5 h), then was purified using the AxyPrep DNA Purification Kit (Axygen, AP-GX-250)

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A biological classification of Parkinsons disease: the SynNeurGe research diagnostic criteria

Separation and purification: Separation and purification is the process of separating the reaction product from the carrier and waste