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A limitation with the use of GLUTag cells, as well as other models for epithelial cells, is that the cells have lost their polarization, which may affect the amino acid sensing mechanisms, as different amino acid transporters are situated on the apical compared to the basolateral membrane [28]

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Br J Nutr 107 , 17871792 (2012)

Some practitioners prefer stacked approaches because healing often involves multiple pathways simultaneously

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