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The (His) 6 -tag was removed by overnight thrombin cleavage, followed by polishing with size-exclusion chromatography (SEC) using a Superdex 75 HiLoad 16/600 column (Cytiva, MA, USA) equilibrated in 20 mM Tris-HCl pH 8.0, and 150 mM NaCl

qiime2R: Importing QIIME2 artifacts and associated data into R sessions

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10.1111/exd.13548 [DOI] [PubMed] [Google Scholar] 6.Yildirim M, Inaloz HS, Baysal V, Delibas N

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Occurrence, Biological Activity and Metabolism of 6-shogaol