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For most people, the body can make enough to meet its needs, but for some people, because of genetic or pharmaceutical reasons, the body cannot produce enough, it is, therefore, an essential nutrient for these individuals (23)

Erba D, Casiraghi MC, Martinez-Conesa C, Goi G, Massaccesi L (2012) Isoflavone supplementation reduces DNA oxidative damage and increases O--N-acetyl-D-glucosaminidase activity in healthy women

Ischemia-Reperfusion Protocol A separate group of hearts were excised as described above and exposed to 30/30 min of global, no-flow ischemia and reperfusion under control conditions (n = 7) or in the presence of 64M 4-ClDzp (n = 5)