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[DOI] [PMC free article] [PubMed] [Google Scholar] Jin T

Binding of FITC-z13 to CNGB3 was measured by adding serially diluted FITC-z13 to CNGB3-expressing HEK293T cells in 96-well plates and incubation at 37 C for 60 min

A melting curve analysis was performed from 60 C to 85 C, and products were run on 2% agar gels to test product specificity using GelRed loading buffer and a pre-stained DNA marker (GenScript, Piscataway, NJ)

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At endpoint, mice were sacrificed and the spleen and all tumors from each individual mouse were resected and weighed