First, isolate your variables
This occurs when the hair roots are attacked by a hormone known as DHT, or simply by aging and stress
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Antibody bound to 8-OH-dG in the cell homogenate is washed away during washing and only antibody bound to the well was detected using a HRP-conjugate and colorimetric substrate and the BMG Fluostar Optima multimode plate reader (NY, USA)
When ascorbate is administered in high doses, it gets oxidized to DHA (Docosahexaenoic acid)
The release of the nitrobenzoic acid group as nitro-2-thiobenzoate anion (TNB ) creates a yellow anion that can be measured spectrophotometrically at a wavelength of 412 nm