3c-d, top strand): template (5-CGGCA rG TACTG-3), upstream primer (5-CAGTAC-3), and 5-phosphorylated downstream primer (5-phosGCCG-3)
Glucocorticoids and endothelial cell barrier function
Cell lines IMR90 (human, American Type Culture Collection (ATCC) CCL-186), SK-MEL-103 (ATCC, HTB-69), A549 (ATCC, CCL-185), MCF7 (ATCC, HTB-22) and PC3 (ATCC, CRL-1435) cells were obtained from the ATCC
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formulated a DSF in-situ gel, which transitions to a gel upon contact with the eye, further retarding cataract development [65]
All treatments were administered via i.p