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Following incubation for 1 h at 37 C, plates were washed with PBST and 3,3',5,5'-Tetramethylbenzidine (TMB Sera care TMB-51200047) was added to all wells and incubated for 5 min at room temperature (RT)

Cycling breaks after this window are recommended on the hypothesis that extended beta-3 adrenergic receptor stimulation leads to receptor downregulation and reduced lipolytic response over time

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The investigators surgically detached the Achilles tendon from its calcaneal insertion in rats an injury that does not heal spontaneously and assessed recovery with and without BPC-157 administration